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CoraLite® Plus 488-conjugated HSP90AB1 Polyclonal antibody
HSP90AB1 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11405-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-11405 targets HSP90AB1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | HSP90AB1 fusion protein Ag1947 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | heat shock protein 90kDa alpha (cytosolic), class B member 1 |
Synonyms | D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB |
Calculated Molecular Weight | 723 aa, 83 kDa |
Observed Molecular Weight | 83-90 kDa |
GenBank Accession Number | BC014485 |
Gene Symbol | HSP90AB1 |
Gene ID (NCBI) | 3326 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08238 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HSP90AB1 antibody CL488-11405 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |