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验证数据展示

  • FC experiment of K-562 using CL647-12361

    FC experiment of K-562 using CL647-12361

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Hemoglobin Epsilon (CL647-12361)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated Hemoglobin Epsilon Polyclonal antibody

Hemoglobin Epsilon Polyclonal Antibody for FC (Intra)
Cat No. CL647-12361

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

HBE1, Hemoglobin epsilon-Specific, Hemoglobin epsilon chain, HBE, Epsilon-globin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 12361-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-12361 targets Hemoglobin Epsilon in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Hemoglobin Epsilon fusion protein Ag3026 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, epsilon 1
别名 HBE1, Hemoglobin epsilon-Specific, Hemoglobin epsilon chain, HBE, Epsilon-globin
计算分子量 147 aa, 16 kDa
观测分子量 12-16 kDa
GenBank蛋白编号BC015537
基因名称 HBE1
Gene ID (NCBI) 3046
RRIDAB_2934870
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02100
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Hemoglobin Epsilon antibody CL647-12361Download protocol
Standard Protocols
Click here to view our Standard Protocols