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验证数据展示

  • FC experiment of K-562 using FITC-66151

    FC experiment of K-562 using FITC-66151

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug FITC Anti-Human HBE1-Specific (FITC-66151, Clone:2C11G6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

FITC-conjugated HBE1-Specific Monoclonal antibody

HBE1-Specific Monoclonal Antibody for FC (Intra)
Cat No. FITC-66151

产品说明书

CloneNo. 2C11G6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

Epsilon globin, HBE, HBE1, HBE1-Specific, Hemoglobin epsilon, Hemoglobin epsilon chain, Hemoglobin epsilon-Specific, Hemoglobin subunit epsilon, hemoglobin, epsilon 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-66151 targets HBE1-Specific in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hemoglobin, epsilon 1
别名 Epsilon globin, HBE, HBE1, HBE1-Specific, Hemoglobin epsilon, Hemoglobin epsilon chain, Hemoglobin epsilon-Specific, Hemoglobin subunit epsilon, hemoglobin, epsilon 1
观测分子量 16 kDa
GenBank蛋白编号NM_005330
基因名称 HBE1
Gene ID (NCBI) 3046
RRIDAB_2883828
偶联类型 FITC Fluorescent Dye
最大激发/发射波长498 nm / 526 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02100
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains. This antibody is conjugated with FITC.

实验方案

Product Specific Protocols
FC protocol for FITC HBE1-Specific antibody FITC-66151Download protocol
Standard Protocols
Click here to view our Standard Protocols