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CoraLite® Plus 488-conjugated Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for WB, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10284

Print datasheet

Synonyms

验证数据展示

  • IF Staining of human tonsillitis using CL488-10284

    IF Staining of human tonsillitis using CL488-10284

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Human IgG antibody (CL488-10284) at dilution of 1:200.

  • WB analysis using CL488-10284

    WB analysis using CL488-10284

    Human plasma were subjected to SDS PAGE followed by western blot with CL488-10284 (Human IgG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma
Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 10284-1-AP

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-10284 targets Human IgG in WB, IF-P applications and shows reactivity with human samples.

Tested Applications WB, IF-P Application Description
Cited ApplicationsIF
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Human IgG
Synonyms
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 25 kDa, 50 kDa, 150 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918970
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This rabbit poly clonal antibody recognizes the human IgG.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Human IgG antibody CL488-10284Download protocol
IF protocol for CL Plus 488 Human IgG antibody CL488-10284Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Oxid Med Cell Longev

Adropin Improves Radiation-Induced Myocardial Injury via VEGFR2/PI3K/Akt Pathway

Authors - Bingda Li
View Article