CoraLite® Plus 647-conjugated IBA1 Monoclonal antibody
IBA1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1C6A10
验证数据展示
经过测试的应用
| Positive FC detected in | THP-1 cells |
For other applications, we recommend the unconjugated version of this antibody, 66827-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-66827 targets IBA1 in FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | human |
| Immunogen | IBA1 fusion protein Ag28236 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | allograft inflammatory factor 1 |
| Synonyms | AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1 |
| Calculated Molecular Weight | 17 kDa |
| GenBank Accession Number | BC009474 |
| Gene Symbol | AIF1 |
| Gene ID (NCBI) | 199 |
| ENSEMBL Gene ID | ENSG00000204472 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P55008 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. (20944424). AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. AIF1 plays a role in RAC signaling and in phagocytosis and a role in vascular inflammation. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. It is also as a microglial cell marker.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 IBA1 antibody CL647-66827 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
