验证数据展示
经过测试的应用
Positive FC (Intra) detected in | THP-1 cells |
Positive FC detected in | THP-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66827 targets IBA1 in FC (Intra) applications and shows reactivity with human samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | IBA1 fusion protein Ag28236 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | allograft inflammatory factor 1 |
别名 | AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1 |
计算分子量 | 17 kDa |
GenBank蛋白编号 | BC009474 |
基因名称 | IBA1 |
Gene ID (NCBI) | 199 |
ENSEMBL Gene ID | ENSG00000204472 |
RRID | AB_2935068 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P55008 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. (20944424). AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. AIF1 plays a role in RAC signaling and in phagocytosis and a role in vascular inflammation. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. It is also as a microglial cell marker.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 IBA1 antibody CL647-66827 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |