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FITC-conjugated ICAM-1/CD54 Monoclonal antibody
ICAM-1/CD54 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF-P and More (1)
Conjugate
FITC Fluorescent Dye
CloneNo.
2F9A8
验证数据展示
经过测试的应用
| Positive IF-P detected in | human lung cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
FITC-60299 targets ICAM-1/CD54 in IF-P applications and shows reactivity with human samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Immunogen | ICAM-1/CD54 fusion protein Ag8309 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | intercellular adhesion molecule 1 |
| Synonyms | CD54, ICAM-1, ICAM1, ICAM 1, BB2 |
| Calculated Molecular Weight | 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC015969 |
| Gene Symbol | ICAM-1 |
| Gene ID (NCBI) | 3383 |
| ENSEMBL Gene ID | ENSG00000090339 |
| RRID | AB_2883738 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 498 nm / 526 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05362 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784). This antibody is FITC conjugated ICAM1 mouse monoclonal antibody (600299-1-Ig) which has passed in WB, IHC and FC detection.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FITC ICAM-1/CD54 antibody FITC-60299 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |