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IFIH1/MDA5 Monoclonal antibody, PBS Only

IFIH1/MDA5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D8B11

Cat no : 66770-1-PBS

Print datasheet

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116

验证数据展示

  • IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

    IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFIH1 antibody included
  • IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

    IHC staining of human liver cancer using 66770-1-Ig (same clone as 66770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFIH1 antibody included
  • WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

    WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

  • WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

    WB analysis using 66770-1-Ig (same clone as 66770-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66770-1-Ig (IFIH1/MDA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66770-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.

产品信息

66770-1-PBS targets IFIH1/MDA5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen IFIH1/MDA5 fusion protein Ag17448 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interferon induced with helicase C domain 1
Synonyms Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116
Calculated Molecular Weight 1025 aa, 117 kDa
Observed Molecular Weight 117-125 kDa
GenBank Accession NumberBC111750
Gene Symbol IFIH1
Gene ID (NCBI) 64135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BYX4
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).