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IFT88 Polyclonal antibody

IFT88 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13967-1-AP

Print datasheet

Synonyms

TTC10, TG737, Intraflagellar transport protein 88 homolog



经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cell, MDCK cells, mouse thymus tissue
Positive IP detected inknockout cells and WT cells, HEK-293 cells
Positive IHC detected inmouse heart tissue, human pancreas tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells, C2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13967-1-AP targets IFT88 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, zebrafish
Immunogen IFT88 fusion protein Ag4980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Synonyms TTC10, TG737, Intraflagellar transport protein 88 homolog
Calculated Molecular Weight 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
RRIDAB_2121979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

实验方案

Product Specific Protocols
WB protocol for IFT88 antibody 13967-1-APDownload protocol
IHC protocol for IFT88 antibody 13967-1-APDownload protocol
IF protocol for IFT88 antibody 13967-1-APDownload protocol
IP protocol for IFT88 antibody 13967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.

Authors - Firestone Ari J AJ
mouseWB

Nature

Functional interaction between autophagy and ciliogenesis.

Authors - Pampliega Olatz O
chickenIF

Science

Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.

Authors - Raman M Das
mouseIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
mouseIF

Nat Genet

Complex interactions between genes controlling trafficking in primary cilia.

Authors - Ocbina Polloneal Jymmiel R PJ
humanIF

Nat Genet

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Authors - Carol-Anne Martin