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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated IFT88 Polyclonal antibody

IFT88 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13967

Print datasheet

Synonyms

D13S1056E, DAF19, hTg737, IFT88, RP11 172H24.2, TG737, TPR repeat protein 10, TTC10

验证数据展示

  • IF Staining of MDCK using CL594-13967

    IF Staining of MDCK using CL594-13967

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 IFT88 antibody (CL594-13967) at dilution of 1:200.

  • IF Staining of hTERT-RPE1 using CL594-13967

    IF Staining of hTERT-RPE1 using CL594-13967

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 IFT88 antibody (CL594-13967) at dilution of 1:200, CoraLite® Plus 488 acetylated Tubulin(Lys40) antibody (CL488-66200, Clone: 7E5H8, green).

经过测试的应用

Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 13967-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-13967 targets IFT88 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, Canine
Immunogen IFT88 fusion protein Ag4980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Synonyms D13S1056E, DAF19, hTg737, IFT88, RP11 172H24.2, TG737, TPR repeat protein 10, TTC10
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 88-95 kDa
GenBank Accession NumberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
RRIDAB_2919801
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13099
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

实验方案

Product Specific Protocols
IF protocol for CL594 IFT88 antibody CL594-13967Download protocol
Standard Protocols
Click here to view our Standard Protocols