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验证数据展示

  • IF Staining of MDCK using CL594-13967

    IF Staining of MDCK using CL594-13967

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 IFT88 antibody (CL594-13967) at dilution of 1:200.

  • IF Staining of hTERT-RPE1 using CL594-13967

    IF Staining of hTERT-RPE1 using CL594-13967

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 IFT88 antibody (CL594-13967) at dilution of 1:200, CoraLite® Plus 488 acetylated Tubulin(Lys40) antibody (CL488-66200, Clone: 7E5H8, green).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated IFT88 Polyclonal antibody

IFT88 Polyclonal Antibody for IF/ICC
Cat No. CL594-13967

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, Canine

应用

IF/ICC

别名

D13S1056E, DAF19, hTg737, IFT88, RP11 172H24.2, TG737, TPR repeat protein 10, TTC10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 13967-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-13967 targets IFT88 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, Canine
免疫原 IFT88 fusion protein Ag4980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 88 homolog (Chlamydomonas)
别名 D13S1056E, DAF19, hTg737, IFT88, RP11 172H24.2, TG737, TPR repeat protein 10, TTC10
计算分子量 94 kDa
观测分子量 88-95 kDa
GenBank蛋白编号BC030776
基因名称 IFT88
Gene ID (NCBI) 8100
RRIDAB_2919801
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13099
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

实验方案

Product Specific Protocols
IF protocol for CL594 IFT88 antibody CL594-13967Download protocol
Standard Protocols
Click here to view our Standard Protocols