NeutraKine® IL-12/IL-23 p40 Mouse McAb

NeutraKine® IL-12/IL-23 p40 Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

2A9H6

Cat no : 69006-1-Ig

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Synonyms

IL-12/IL-23 p40, IL12B, IL 12B, IL 12 subunit p40, IL 12 P40

验证数据展示

Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig
Recombinant human IL-23 (Cat.NO. HZ-1254) induces invitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL.
Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig
Recombinant human IL-23 (Cat.NO. HZ-1254) induces in vitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL. The NeutraControl mouse anti-human IL-23 p40 monoclonal antibody 69506-1-Ig could recognize human IL-23, but could not neutralize human IL-23 in the range of 0.244-4000ng/mL(green curve, refer to top X-right Y axis).
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Recombinant human IL-23 (Cat.NO. HZ-1254) induces in vitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL. The NeutraControl mouse anti-human IL-23 p40 monoclonal antibody 69506-1-Ig could recognize human IL-23, but could not neutralize human IL-23 in the range of 0.244-4000ng/mL(green curve, refer to top X-right Y axis).
ELISA experiment of Recombinant protein using 69006-1-Ig
Indirect ELISA was carried out by coating recombinant Human IL-23 (Cat.NO. HZ-1254) at 70 ng/well followed by blocking and adding serial diluted IL-23 antibody 69006-1-Ig and 69506-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.
ELISA experiment of Recombinant protein using 69006-1-Ig
Indirect ELISA was carried out by coating recombinant Human IL-12 (Cat.NO. HZ-1256) and IL-12 p40 (Cat.NO. HZ-1321) respectively at 70 ng/well followed by blocking and adding serial diluted 69029-1-Ig, 69529-1-Ig and 69006-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggests that 69029-1-Ig and 69529-1-Ig only recognize IL-12(p35 and p40 heterodimer) but not recognize IL-12/IL-23 p40.
×
Indirect ELISA was carried out by coating recombinant Human IL-12 (Cat.NO. HZ-1256) and IL-12 p40 (Cat.NO. HZ-1321) respectively at 70 ng/well followed by blocking and adding serial diluted 69029-1-Ig, 69529-1-Ig and 69006-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggests that 69029-1-Ig and 69529-1-Ig only recognize IL-12(p35 and p40 heterodimer) but not recognize IL-12/IL-23 p40.

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Cat No. 69006-1-Ig

Size

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Neutralization

产品信息

69006-1-Ig targets NeutraKine® IL-12/IL-23 p40 in Neutralization, ELISA applications and shows reactivity with human samples.

Tested Applications	Neutralization, ELISA Application Description
Tested Reactivity	human
Immunogen	human Humankine IL-12/IL-23 p40 protein HZ-1254 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Synonyms	IL-12/IL-23 p40, IL12B, IL 12B, IL 12 subunit p40, IL 12 P40
Calculated Molecular Weight	328 aa, 37 kDa
GenBank Accession Number	BC067498
Gene Symbol	IL12B
Gene ID (NCBI)	51561,3593
RRID	AB_2882924
Conjugate	Unconjugated
Form	Lyophilized Powder
Purification Method	Protein A purification
UNIPROT ID	P29460
Storage Buffer	Sterile PBS.
Endotoxin	<0.1 EU/μg
Reconstitution	This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and Storage	Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

This antibody can be used to neutralize the bioactivity of IL-12/IL-23 p40.