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IRF3 Polyclonal antibody

IRF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (4)

Conjugate

Unconjugated

Publications

185

Cat no : 11312-1-AP

Print datasheet

Synonyms

IRF-3, IRF 3, interferon regulatory factor 3

验证数据展示

WB analysis of HeLa using 11312-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat cells using 11312-1-AP
WB result of IRF3 specific antibody (11312-1-AP, 1:5000) with si-control and si-IRF3 transfected Jurkat cells.
IP experiment of Jurkat using 11312-1-AP
IP result of anti-IRF3 (IP:11312-1-AP, 3ug; Detection:11312-1-AP 1:1000) with Jurkat cells lysate 2400ug.
IHC staining of human spleen using 11312-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with IRF3 antibody included
WB analysis using 11312-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 11312-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 11312-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 11312-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IRF3 antibody (11312-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Cat No. 11312-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293T cells, HCT 116 cells, HeLa cells, U-937 cells, human brain tissue, Jurkat cells, MCF-7 cells, MDA-MB-231 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 170 publications below
IHC	See 2 publications below
IF	See 32 publications below
IP	See 7 publications below
CoIP	See 3 publications below
ChIP	See 2 publications below
RIP	See 1 publications below

产品信息

11312-1-AP targets IRF3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, ChIP, RIP
Tested Reactivity	human
Cited Reactivity	human, rat, pig, monkey, bovine, hamster, sheep
Immunogen	IRF3 fusion protein Ag1858 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	interferon regulatory factor 3
Synonyms	IRF-3, IRF 3, interferon regulatory factor 3
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	50-60 kDa
GenBank Accession Number	BC009395
Gene Symbol	IRF3
Gene ID (NCBI)	3661
RRID	AB_2127004
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14653
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.

实验方案

Product Specific Protocols
WB protocol for IRF3 antibody 11312-1-AP	Download protocol
IHC protocol for IRF3 antibody 11312-1-AP	Download protocol
IF protocol for IRF3 antibody 11312-1-AP	Download protocol
IP protocol for IRF3 antibody 11312-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	ChIP	Mol Cancer YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment Authors - Zhenyun Yang View Article
human	WB	Cell The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death. Authors - Christian Zierhut View Article
human	WB,IF	Nat Immunol The tumor suppressor PTEN has a critical role in antiviral innate immunity. Authors - Shun Li View Article
		Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation Authors - Stephanie Pei Tung Yiu View Article
human	WB	Cell Host Microbe PMID: 29478775 Authors - Jiazheng Xie View Article
human	WB,IF	Acta Pharm Sin B Allosteric inhibition reveals SHP2-mediated tumor immunosuppression in colon cancer by single-cell transcriptomics. Authors - Jian Gao View Article

IRF3 Polyclonal antibody

IRF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis of HeLa using 11312-1-AP

WB analysis of Jurkat cells using 11312-1-AP

IP experiment of Jurkat using 11312-1-AP

IHC staining of human spleen using 11312-1-AP

WB analysis using 11312-1-AP

WB analysis using 11312-1-AP

WB analysis using 11312-1-AP

IF Staining of HeLa using 11312-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment

The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death.

The tumor suppressor PTEN has a critical role in antiviral innate immunity.

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

PMID: 29478775

Allosteric inhibition reveals SHP2-mediated tumor immunosuppression in colon cancer by single-cell transcriptomics.

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