• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 11312-1-AP (same clone as 11312-1-PBS)

    WB analysis of HeLa using 11312-1-AP (same clone as 11312-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat cells using 11312-1-AP (same clone as 11312-1-PBS)

    WB analysis of Jurkat cells using 11312-1-AP (same clone as 11312-1-PBS)

    WB result of IRF3 specific antibody (11312-1-AP, 1:5000) with si-control and si-IRF3 transfected Jurkat cells. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 11312-1-AP (same clone as 11312-1-PBS)

    IP experiment of Jurkat using 11312-1-AP (same clone as 11312-1-PBS)

    IP result of anti-IRF3 (IP:11312-1-AP, 3ug; Detection:11312-1-AP 1:1000) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 11312-1-AP (same clone as 11312-1-PBS)

    IHC staining of human spleen using 11312-1-AP (same clone as 11312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 11312-1-AP (IRF3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF3 antibody included
  • WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11312-1-AP (same clone as 11312-1-PBS)

    IF Staining of HeLa using 11312-1-AP (same clone as 11312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IRF3 antibody (11312-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IRF3 Polyclonal antibody, PBS Only

IRF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11312-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

interferon regulatory factor 3, IRF 3, IRF-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

产品信息

11312-1-PBS targets IRF3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 IRF3 fusion protein Ag1858 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon regulatory factor 3
别名 interferon regulatory factor 3, IRF 3, IRF-3
计算分子量 47 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC009395
基因名称 IRF3
Gene ID (NCBI) 3661
RRIDAB_2127004
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14653
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.