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IRF9 Polyclonal antibody

IRF9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14167-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

ISGF3G, ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, mouse heart tissue, rat heart tissue, HepG2 cells, MCF-7 cells, HeLa cells, THP-1 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IRF9 Ready-To-Use IHC Kit. IRF9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14167-1-AP targets IRF9 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, sheep
免疫原 IRF9 fusion protein Ag5365 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IRF 9
别名 ISGF3G, ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma
计算分子量 393 aa, 44 kDa
观测分子量 44-48 kDa
GenBank蛋白编号BC035716
基因名称 IRF9
Gene ID (NCBI) 10379
RRIDAB_2296227
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00978
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.

实验方案

Product Specific Protocols
WB protocol for IRF9 antibody 14167-1-APDownload protocol
IHC protocol for IRF9 antibody 14167-1-APDownload protocol
IF protocol for IRF9 antibody 14167-1-APDownload protocol
IP protocol for IRF9 antibody 14167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
mouseWB

Cell Rep

miR-342-5p decreases ankyrin G levels in Alzheimer's disease transgenic mouse models.

Authors - Xiaqin Sun
ChIP

PLoS Pathog

A host cell long noncoding RNA NR_033736 regulates type I interferon-mediated gene transcription and modulates intestinal epithelial anti-Cryptosporidium defense.

Authors - Juan Li

Front Immunol

PMID: 30158934

Authors - Ming-Hsun Tsai
humanWB

Mol Cancer Ther

JAK2 inhibitor SAR302503 abrogates PD-L1 expression and targets therapy resistant non-small cell lung cancers.

Authors - Sean Pitroda
humanWB, IP, IF

J Immunol

Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.

Authors - Mudan Zhang
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