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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IRS1 Polyclonal antibody

IRS1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17509

Print datasheet

Synonyms

IRS-1, IRS 1, insulin receptor substrate 1, HIRS 1

验证数据展示

  • IF Staining of A549 using CL488-17509

    IF Staining of A549 using CL488-17509

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 IRS1 antibody (CL488-17509) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-17509

    FC experiment of MCF-7 using CL488-17509

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human IRS1 (CL488-17509) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 17509-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17509 targets IRS1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen IRS1 fusion protein Ag11714 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name insulin receptor substrate 1
Synonyms IRS-1, IRS 1, insulin receptor substrate 1, HIRS 1
Calculated Molecular Weight 1242 aa, 132 kDa
Observed Molecular Weight 160-185 kDa
GenBank Accession NumberBC053895
Gene Symbol IRS1
Gene ID (NCBI) 3667
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35568
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IRS1 antibody CL488-17509Download protocol
Standard Protocols
Click here to view our Standard Protocols