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验证数据展示

  • IF Staining of A549 using CL488-17509

    IF Staining of A549 using CL488-17509

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 IRS1 antibody (CL488-17509) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-17509

    FC experiment of MCF-7 using CL488-17509

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human IRS1 (CL488-17509) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated IRS1 Polyclonal antibody

IRS1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-17509

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

IRS-1, IRS 1, insulin receptor substrate 1, HIRS 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 17509-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17509 targets IRS1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 IRS1 fusion protein Ag11714 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin receptor substrate 1
别名 IRS-1, IRS 1, insulin receptor substrate 1, HIRS 1
计算分子量 1242 aa, 132 kDa
观测分子量 160-185 kDa
GenBank蛋白编号BC053895
基因名称 IRS1
Gene ID (NCBI) 3667
RRIDAB_3084006
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35568
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IRS1 antibody CL488-17509Download protocol
Standard Protocols
Click here to view our Standard Protocols