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KRAS-2A-specific Polyclonal antibody

KRAS-2A-specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 16156-1-AP

Print datasheet

Synonyms

KRAS-2A, KRAS, KRAS4A, KRAS4B, pan RAS

验证数据展示

  • WB analysis of NIH/3T3 using 16156-1-AP

    WB analysis of NIH/3T3 using 16156-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 16156-1-AP

    WB analysis using 16156-1-AP

    Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 16156-1-AP

    FC experiment of HeLa using 16156-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS-2A-specific (16156-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach using 16156-1-AP

    IHC staining of human stomach using 16156-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16156-1-AP (KRAS-2A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16156-1-AP

    IF Staining of HeLa using 16156-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KRAS-2A-specific antibody (16156-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16156-1-AP targets KRAS-2A-specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS-2A, KRAS, KRAS4A, KRAS4B, pan RAS
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberNM_033360
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2234477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .

实验方案

Product Specific Protocols
WB protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
IHC protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
IF protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Authors - Sascha Röth
View Article
humanWB

Sci Signal

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Authors - Andrew M Waters
View Article
humanWB

Life Sci Alliance

A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95

Authors - Kristen M Rehl
View Article