验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66607 targets KU70,XRCC6 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | KU70,XRCC6 fusion protein Ag21301 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Synonyms | KU70, XRCC6, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC008343 |
| Gene Symbol | KU70 |
| Gene ID (NCBI) | 2547 |
| RRID | AB_2934736 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P12956 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 KU70,XRCC6 antibody CL594-66607 | Download protocol |
| IF protocol for CL594 KU70,XRCC6 antibody CL594-66607 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
