验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66607 targets KU70,XRCC6 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | KU70,XRCC6 fusion protein Ag21301 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| 别名 | KU70, XRCC6, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1 |
| 计算分子量 | 75 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC008343 |
| 基因名称 | KU70 |
| Gene ID (NCBI) | 2547 |
| RRID | AB_2934736 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P12956 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 KU70,XRCC6 antibody CL594-66607 | Download protocol |
| IF protocol for CL594 KU70,XRCC6 antibody CL594-66607 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
