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CoraLite®594-conjugated KU70,XRCC6 Monoclonal antibody

KU70,XRCC6 Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C12D9

Cat no : CL594-66607

Print datasheet

Synonyms

KU70, XRCC6, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1

验证数据展示

  • WB analysis using CL594-66607

    WB analysis using CL594-66607

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66607 (KU70,XRCC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using CL594-66607

    IF Staining of HeLa using CL594-66607

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KU70,XRCC6 antibody (CL594-66607, Clone: 3C12D9 ) at dilution of 1:600.

  • FC experiment of HepG2 using CL594-66607

    FC experiment of HepG2 using CL594-66607

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KU70,XRCC6 (CL594-66607, Clone:3C12D9) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy XRCC6 Ready-To-Use IHC Kit. XRCC6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66607-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66607 targets KU70,XRCC6 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen KU70,XRCC6 fusion protein Ag21301 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Synonyms KU70, XRCC6, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC008343
Gene Symbol KU70
Gene ID (NCBI) 2547
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP12956
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

实验方案

Product Specific Protocols
WB protocol for CL594 KU70,XRCC6 antibody CL594-66607Download protocol
IF protocol for CL594 KU70,XRCC6 antibody CL594-66607Download protocol
Standard Protocols
Click here to view our Standard Protocols