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CoraLite® Plus 488-conjugated L-VOCC Polyclonal antibody

L-VOCC Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21774

Print datasheet

Synonyms

CACNA1C, CCHL1A1, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, CACNL1A1, CACN2

验证数据展示

  • IF Staining of mouse brain using CL488-21774

    IF Staining of mouse brain using CL488-21774

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 L-VOCC antibody (CL488-21774) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL488-21774

    IF Staining of mouse brain using CL488-21774

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 L-VOCC antibody (CL488-21774) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using CL488-21774

    FC experiment of HeLa using CL488-21774

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human L-VOCC (CL488-21774) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 21774-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21774 targets L-VOCC in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen L-VOCC fusion protein Ag16455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms CACNA1C, CCHL1A1, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, CACNL1A1, CACN2
Calculated Molecular Weight 2135 aa, 239 kDa
GenBank Accession NumberBC146846
Gene Symbol CACNA1C
Gene ID (NCBI) 775
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13936
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 L-VOCC antibody CL488-21774Download protocol
FC protocol for CL Plus 488 L-VOCC antibody CL488-21774Download protocol
Standard Protocols
Click here to view our Standard Protocols