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验证数据展示

  • IF Staining of A549 using CL488-68080

    IF Staining of A549 using CL488-68080

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

  • IF Staining of A549 using CL488-68080

    IF Staining of A549 using CL488-68080

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

  • FC experiment of A549 using CL488-68080

    FC experiment of A549 using CL488-68080

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human LASP1 (CL488-68080, Clone:1G4B6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated LASP1 Monoclonal antibody

LASP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68080

产品说明书

CloneNo. 1G4B6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

MLN50, LIM and SH3 protein 1, LASP-1, 1G4B6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68080-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68080 targets LASP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 LASP1 fusion protein Ag18101 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LIM and SH3 protein 1
别名 MLN50, LIM and SH3 protein 1, LASP-1, 1G4B6
计算分子量 30 kDa
观测分子量35-38 kDa
GenBank蛋白编号BC012460
基因名称 LASP1
Gene ID (NCBI) 3927
ENSEMBL Gene IDENSG00000002834
RRIDAB_2934619
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14847
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LASP1 antibody CL488-68080Download protocol
FC protocol for CL Plus 488 LASP1 antibody CL488-68080Download protocol
Standard Protocols
Click here to view our Standard Protocols