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KD/KO Validated

LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Publications

247

Cat no : 19987-1-AP

Print datasheet

Synonyms

LDHA, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

验证数据展示

WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 19987-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 19987-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP experiment of mouse kidney using 19987-1-AP
IP result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.
IHC staining of human heart using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 19987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).
WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 19987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19987-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Cat No. 19987-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human heart tissue, human skeletal muscle tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 12 publications below
WB	See 218 publications below
IHC	See 39 publications below
IF	See 13 publications below
IP	See 6 publications below
FC	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

产品信息

19987-1-AP targets LDHA-Specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, chicken, goat
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	lactate dehydrogenase A
Synonyms	LDHA, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	32-37 kDa
GenBank Accession Number	NM_005566
Gene Symbol	LDHA
Gene ID (NCBI)	3939
RRID	AB_10646429
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00338
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

实验方案

Product Specific Protocols
WB protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IHC protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IF protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
IP protocol for LDHA-Specific antibody 19987-1-AP	Download protocol
FC protocol for LDHA-Specific antibody 19987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
mouse	WB	Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis Authors - View Article
human	WB	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li View Article
human	WB	Drug Resist Updat Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression Authors - Shuai Wang KD Validated View Article
human		Cell Stem Cell Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury Authors - Shuyong Wang View Article
human		Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. Authors - Di Huang View Article

LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis using 19987-1-AP

IF Staining of HepG2 using 19987-1-AP

IF Staining of HepG2 using 19987-1-AP

IP experiment of mouse kidney using 19987-1-AP

IHC staining of human heart using 19987-1-AP

IHC staining of human heart using 19987-1-AP

WB analysis using 19987-1-AP

WB analysis using 19987-1-AP

WB analysis of HeLa using 19987-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Acetate enables metabolic fitness and cognitive performance during sleep disruption

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression

Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

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