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验证数据展示

  • IF Staining of HepG2 using CL647-19987

    IF Staining of HepG2 using CL647-19987

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 647 LDHA-Specific antibody (CL647-19987) at dilution of 1:200.

  • FC experiment of HepG2 using CL647-19987

    FC experiment of HepG2 using CL647-19987

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human LDHA-Specific (CL647-19987) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-19987

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

LDHA, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 19987-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-19987 targets LDHA-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lactate dehydrogenase A
Synonyms LDHA, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberNM_005566
Gene Symbol LDHA
Gene ID (NCBI) 3939
RRIDAB_2934939
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00338
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 LDHA-Specific antibody CL647-19987Download protocol
FC protocol for CL Plus 647 LDHA-Specific antibody CL647-19987Download protocol
Standard Protocols
Click here to view our Standard Protocols