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LONP1 Monoclonal antibody, PBS Only

LONP1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C12

Cat No : 66043-1-PBS

Print datasheet

Synonyms

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

验证数据展示

  • WB analysis using 66043-1-Ig (same clone as 66043-1-PBS)

    WB analysis using 66043-1-Ig (same clone as 66043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of C6 cells using 66043-1-Ig (LONP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using LONP1 antibody (66043-1-Ig, Clone: 1C6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

产品信息

66043-1-PBS targets LONP1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LONP1 fusion protein Ag7306 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15
Calculated Molecular Weight 106 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36776
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).