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LONP1 Monoclonal antibody

LONP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1C6C12

Cat no : 66043-1-Ig

Print datasheet

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53



经过测试的应用

Positive WB detected inROS1728 cells, HEK-293 cells, HSC-T6 cells, human heart tissue, human liver tissue, Jurkat cells, L02 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66043-1-Ig targets LONP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen LONP1 fusion protein Ag7306 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53
Calculated Molecular Weight 106 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2881494
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

实验方案

Product Specific Protocols
WB protocol for LONP1 antibody 66043-1-IgDownload protocol
IHC protocol for LONP1 antibody 66043-1-IgDownload protocol
IF protocol for LONP1 antibody 66043-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanCoIP

Acta Pharm Sin B

Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication

Authors - Xiaowei Wang
humanWB

EMBO J

Stress-dependent macromolecular crowding in the mitochondrial matrix

Authors - Elianne P Bulthuis
human,mouseIHC,WB

Mol Med

ATF5 regulates tubulointerstitial injury in diabetic kidney disease via mitochondrial unfolded protein response

Authors - Yifei Liu
mouseWB

J Physiol Biochem

PGC-1α activation boosts exercise-dependent cellular response in the skeletal muscle

Authors - Soroosh Mozaffaritabar
humanIF

Antioxid Redox Signal

Regulation of Erythroid Differentiation via the HIF1α-NFIL3-PIM1 Signaling Axis Under Hypoxia

Authors - Hai-Chuan Yu
humanWB

Mol Cell

A mitochondrial iron-responsive pathway regulated by DELE1

Authors - Yusuke Sekine
  • KD Validated