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CoraLite® Plus 488-conjugated LONP1 Monoclonal antibody

LONP1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66043

产品说明书

CloneNo. 1C6C12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inC6 cells
For other applications, we recommend the unconjugated version of this antibody, 66043-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66043 targets LONP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 LONP1 fusion protein Ag7306 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lon peptidase 1, mitochondrial
别名 hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15
计算分子量 106 kDa
观测分子量 100 kDa
GenBank蛋白编号BC000235
基因名称 LONP1
Gene ID (NCBI) 9361
RRIDAB_2919281
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP36776
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LONP1 antibody CL488-66043Download protocol
Standard Protocols
Click here to view our Standard Protocols
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