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验证数据展示

  • IF Staining of C6 using CL594-66043

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100. DAPI (blue).

  • IF Staining of C6 using CL594-66043

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-Phalloidin (green).

  • IF Staining of C6 using CL594-66043

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-Phalloidin (green).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated LONP1 Monoclonal antibody

LONP1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66043

Print datasheet

CloneNo. 1C6C12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inC6 cells
For other applications, we recommend the unconjugated version of this antibody, 66043-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66043 targets LONP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen LONP1 fusion protein Ag7306 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53
Calculated Molecular Weight 106 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2919942
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36776
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

实验方案

Product Specific Protocols
IF protocol for CL594 LONP1 antibody CL594-66043Download protocol
Standard Protocols
Click here to view our Standard Protocols