TAU Polyclonal antibody
TAU Polyclonal Antibody for WB, IHC, IF-Fro, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-Fro, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain |
Positive IHC detected in | human brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-Fro detected in | mouse brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10274-1-AP targets TAU in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-Fro, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat |
Immunogen | TAU fusion protein Ag0354 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | microtubule-associated protein tau |
Synonyms | MAPT, Paired helical filament tau, Microtubule-associated protein tau, MAPTL, DDPAC |
Calculated Molecular Weight | 37-46, 79-81 kDa |
Observed Molecular Weight | 45-70 kDa, 100 kDa |
GenBank Accession Number | BC000558 |
Gene Symbol | TAU |
Gene ID (NCBI) | 4137 |
RRID | AB_2139718 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P10636 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TAU antibody 10274-1-AP | Download protocol |
IHC protocol for TAU antibody 10274-1-AP | Download protocol |
IF protocol for TAU antibody 10274-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease. | ||
Hum Mol Genet Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice. | ||
Acta Neuropathol Commun Laterality and region-specific tau phosphorylation correlate with PTSD-related behavioral traits in rats exposed to repetitive low-level blast. | ||
Free Radic Biol Med The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro | ||
Mol Ther Nucleic Acids Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease. |