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验证数据展示

  • IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

    IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

    IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    WB analysis of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

TAU Polyclonal antibody, PBS Only

TAU Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 10274-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, ELISA

别名

MAPT, Paired helical filament tau, Microtubule-associated protein tau, MAPTL, DDPAC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

10274-1-PBS targets TAU in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TAU fusion protein Ag0354 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein tau
别名 MAPT, Paired helical filament tau, Microtubule-associated protein tau, MAPTL, DDPAC
计算分子量 37-46, 79-81 kDa
观测分子量 45-70 kDa, 100 kDa
GenBank蛋白编号BC000558
基因名称 TAU
Gene ID (NCBI) 4137
RRIDAB_2139718
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10636
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.