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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MCM6 Polyclonal antibody

MCM6 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13347

Print datasheet

Synonyms

MCG40308, MCM6, Mis5, P105MCM

验证数据展示

  • IF Staining of HeLa using CL488-13347

    IF Staining of HeLa using CL488-13347

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MCM6 antibody (CL488-13347) at dilution of 1:200, CL594-phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MCM6 Ready-To-Use IHC Kit. MCM6 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13347 targets MCM6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM6 fusion protein Ag4180 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name minichromosome maintenance complex component 6
Synonyms MCG40308, MCM6, Mis5, P105MCM
Calculated Molecular Weight 821 aa, 93 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC032374
Gene Symbol MCM6
Gene ID (NCBI) 4175
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14566
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCM6 antibody CL488-13347Download protocol
Standard Protocols
Click here to view our Standard Protocols