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验证数据展示

  • IF Staining of HeLa using CL488-13347

    IF Staining of HeLa using CL488-13347

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MCM6 antibody (CL488-13347) at dilution of 1:200, CL594-phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated MCM6 Polyclonal antibody

MCM6 Polyclonal Antibody for IF/ICC
Cat No. CL488-13347

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

MCG40308, MCM6, Mis5, P105MCM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MCM6 Ready-To-Use IHC Kit. MCM6 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13347 targets MCM6 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 MCM6 fusion protein Ag4180 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 6
别名 MCG40308, MCM6, Mis5, P105MCM
计算分子量 821 aa, 93 kDa
观测分子量 105 kDa
GenBank蛋白编号BC032374
基因名称 MCM6
Gene ID (NCBI) 4175
RRIDAB_3083937
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14566
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCM6 antibody CL488-13347Download protocol
Standard Protocols
Click here to view our Standard Protocols