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CoraLite® Plus 488-conjugated MCM7 Polyclonal antibody

MCM7 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11225

Print datasheet

Synonyms

P1.1 MCM3, MCM2, EC:3.6.4.12, CDC47 homolog, CDC47

验证数据展示

  • IF Staining of A431 using CL488-11225

    IF Staining of A431 using CL488-11225

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MCM7 antibody (CL488-11225) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-11225

    FC experiment of HepG2 using CL488-11225

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MCM7 (CL488-11225) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCM7 Ready-To-Use IHC Kit. MCM7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11225-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11225 targets MCM7 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM7 fusion protein Ag1730 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name minichromosome maintenance complex component 7
Synonyms P1.1 MCM3, MCM2, EC:3.6.4.12, CDC47 homolog, CDC47
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC013375
Gene Symbol MCM7
Gene ID (NCBI) 4176
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33993
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCM7 antibody CL488-11225Download protocol
Standard Protocols
Click here to view our Standard Protocols