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验证数据展示

  • WB analysis of pig brain using 66527-1-Ig (same clone as 66527-1-PBS)

    WB analysis of pig brain using 66527-1-Ig (same clone as 66527-1-PBS)

    pig/rat/mouse brain tissues were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IF Staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFF antibody (66527-1-Ig, Clone: 1G4B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

MFF Monoclonal antibody, PBS Only

MFF Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66527-1-PBS

产品说明书

CloneNo. 1G4B12

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rat, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

AD033, C2orf33, MFF, mitochondrial fission factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66527-1-PBS targets MFF in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, pig, rat, mouse
免疫原 MFF fusion protein Ag9990 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial fission factor
别名 AD033, C2orf33, MFF, mitochondrial fission factor
计算分子量 38 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC000797
基因名称 MFF
Gene ID (NCBI) 56947
RRIDAB_2881890
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9GZY8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.