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MFF Monoclonal antibody, PBS Only

MFF Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4B12

Cat no : 66527-1-PBS

Print datasheet

Synonyms

AD033, C2orf33, MFF, mitochondrial fission factor

验证数据展示

  • WB analysis of pig brain using 66527-1-Ig (same clone as 66527-1-PBS)

    WB analysis of pig brain using 66527-1-Ig (same clone as 66527-1-PBS)

    pig/rat/mouse brain tissues were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IF Staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFF antibody (66527-1-Ig, Clone: 1G4B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    IHC staining of mouse brain using 66527-1-Ig (same clone as 66527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66527-1-PBS in a different storage buffer formulation.

产品信息

66527-1-PBS targets MFF in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, pig, rat, mouse
Immunogen MFF fusion protein Ag9990 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitochondrial fission factor
Synonyms AD033, C2orf33, MFF, mitochondrial fission factor
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC000797
Gene Symbol MFF
Gene ID (NCBI) 56947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZY8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.