MFF Monoclonal antibody

MFF Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G4B12

Cat No : 66527-1-Ig

Print datasheet

Synonyms

AD033, C2orf33, MFF, mitochondrial fission factor



经过测试的应用

Positive WB detected inpig brain tissue, L02 cells, HepG2 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66527-1-Ig targets MFF in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications WB
Tested Reactivity Human, pig, rat, mouse
Cited Reactivitymouse, rat
Immunogen MFF fusion protein Ag9990 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitochondrial fission factor
Synonyms AD033, C2orf33, MFF, mitochondrial fission factor
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC000797
Gene Symbol MFF
Gene ID (NCBI) 56947
RRIDAB_2881890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

实验方案

Product Specific Protocols
WB protocol for MFF antibody 66527-1-IgDownload protocol
IHC protocol for MFF antibody 66527-1-IgDownload protocol
IF protocol for MFF antibody 66527-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Phytother Res

Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow

Authors - Jing-Jing Li
mouseWB

J Adv Res

Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics

Authors - Liuyang Tian