验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
For other applications, we recommend the unconjugated version of this antibody, 66527-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66527 targets MFF in IF-P applications and shows reactivity with Human, pig, rat, mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, pig, rat, mouse |
| 免疫原 | MFF fusion protein Ag9990 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial fission factor |
| 别名 | AD033, C2orf33, DKFZp666J168, GL004, MFF, mitochondrial fission factor |
| 计算分子量 | 38 kDa |
| GenBank蛋白编号 | BC000797 |
| 基因名称 | MFF |
| Gene ID (NCBI) | 56947 |
| RRID | AB_2920002 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9GZY8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MFF antibody CL594-66527 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |