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CoraLite®594-conjugated MFF Monoclonal antibody

MFF Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G4B12

Cat no : CL594-66527

Print datasheet

Synonyms

AD033, C2orf33, DKFZp666J168, GL004, MFF, mitochondrial fission factor

验证数据展示

  • IF Staining of mouse brain using CL594-66527

    IF Staining of mouse brain using CL594-66527

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 MFF antibody (CL594-66527, Clone: 1G4B12 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 66527-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66527 targets MFF in IF-P applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, pig, rat, mouse
Immunogen MFF fusion protein Ag9990 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitochondrial fission factor
Synonyms AD033, C2orf33, DKFZp666J168, GL004, MFF, mitochondrial fission factor
Calculated Molecular Weight 38 kDa
GenBank Accession NumberBC000797
Gene Symbol MFF
Gene ID (NCBI) 56947
RRIDAB_2920002
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZY8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 MFF antibody CL594-66527Download protocol
Standard Protocols
Click here to view our Standard Protocols