验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17280-1-AP targets MRPS27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MRPS27 fusion protein Ag11047 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial ribosomal protein S27 |
| 别名 | Small ribosomal subunit protein mS27, S27mt, MRP-S27, MRPS 27, MRP S27 |
| 计算分子量 | 168 aa, 19 kDa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC030521 |
| 基因名称 | MRPS27 |
| Gene ID (NCBI) | 23107 |
| RRID | AB_2180510 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92552 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPS27 antibody 17280-1-AP | Download protocol |
| IHC protocol for MRPS27 antibody 17280-1-AP | Download protocol |
| IF protocol for MRPS27 antibody 17280-1-AP | Download protocol |
| IP protocol for MRPS27 antibody 17280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. | ||
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
J Clin Invest AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival. | ||
J Cell Biol Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. | ||
Proc Natl Acad Sci U S A Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription. |
