验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Caco-2 cells, SW480 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15520-1-AP targets MSH2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | MSH2 fusion protein Ag7835 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) |
| Synonyms | MSH 2 |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 100 kDa-105 kDa |
| GenBank Accession Number | BC021566 |
| Gene Symbol | MSH2 |
| Gene ID (NCBI) | 4436 |
| RRID | AB_2144799 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MSH2 antibody 15520-1-AP | Download protocol |
| IHC protocol for MSH2 antibody 15520-1-AP | Download protocol |
| IF protocol for MSH2 antibody 15520-1-AP | Download protocol |
| IP protocol for MSH2 antibody 15520-1-AP | Download protocol |
| FC protocol for MSH2 antibody 15520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Leukemia Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia
| ||
J Immunother Cancer KALRN mutations promote antitumor immunity and immunotherapy response in cancer. | ||
Hum Mol Genet Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.
| ||
Cancer Prev Res (Phila) Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells. | ||
Front Oncol Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence. | ||
J Biol Chem Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways. |
