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  • Featured Product
  • KD/KO Validated

MSH2 Polyclonal antibody

MSH2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15520-1-AP

Print datasheet

Synonyms

MSH 2

验证数据展示

  • WB analysis of HeLa using 15520-1-AP

    WB analysis of HeLa using 15520-1-AP

    WB results of MSH2 (15520-1-AP, 1:5000) antibody with si-Control and si-MSH2 transfected HeLa cells..

  • WB analysis using 15520-1-AP

    WB analysis using 15520-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15520-1-AP

    IHC staining of human stomach cancer using 15520-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human stomach cancer using 15520-1-AP

    IHC staining of human stomach cancer using 15520-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH2 antibody included
  • IF Staining of HeLa using 15520-1-AP

    IF Staining of HeLa using 15520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody (15520-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 15520-1-AP

    IP experiment of HeLa using 15520-1-AP

    IP result of anti-MSH2 (IP:15520-1-AP, 5ug; Detection:15520-1-AP 1:500) with HeLa cells lysate 2000ug.

  • FC experiment of HeLa using 15520-1-AP

    FC experiment of HeLa using 15520-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MSH2 (15520-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, Caco-2 cells, SW480 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human tonsil tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15520-1-AP targets MSH2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MSH2 fusion protein Ag7835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Synonyms MSH 2
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100 kDa-105 kDa
GenBank Accession NumberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
RRIDAB_2144799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43246
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

实验方案

Product Specific Protocols
WB protocol for MSH2 antibody 15520-1-APDownload protocol
IHC protocol for MSH2 antibody 15520-1-APDownload protocol
IF protocol for MSH2 antibody 15520-1-APDownload protocol
IP protocol for MSH2 antibody 15520-1-APDownload protocol
FC protocol for MSH2 antibody 15520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Leukemia

Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia

Authors - Ji-Yuan Teng
  • KO Validated
View Article
humanWB

J Immunother Cancer

KALRN mutations promote antitumor immunity and immunotherapy response in cancer.

Authors - Mengyuan Li
View Article
humanWB, IHC

Hum Mol Genet

Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.

Authors - Dazhang Bai
  • KD Validated
View Article
humanWB

Cancer Prev Res (Phila)

Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.

Authors - Jin Peng P
View Article
humanIHC

Front Oncol

Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.

Authors - Xuting Ran
View Article
humanWB

J Biol Chem

Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways.

Authors - Mo Chen C
View Article