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验证数据展示

  • IF Staining of HeLa using CL488-15520

    IF Staining of HeLa using CL488-15520

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MSH2 antibody (CL488-15520) at dilution of 1:400, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-15520

    FC experiment of HeLa using CL488-15520

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MSH2 (CL488-15520) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MSH2 Polyclonal antibody

MSH2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15520

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

MSH 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15520-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15520 targets MSH2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 MSH2 fusion protein Ag7835 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
别名 MSH 2
计算分子量 105 kDa
观测分子量 100 kDa-105 kDa
GenBank蛋白编号BC021566
基因名称 MSH2
Gene ID (NCBI) 4436
RRIDAB_3083972
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43246
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MSH2 antibody CL488-15520Download protocol
FC protocol for CL Plus 488 MSH2 antibody CL488-15520Download protocol
Standard Protocols
Click here to view our Standard Protocols