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AEG-1/MTDH Polyclonal antibody
AEG-1/MTDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, A375 cells, HeLa cells, DU 145 cells, HL-60 cells, A549 cells, mouse spleen tissue, PC-3 cells, HepG2 cells, RAW 264.7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy MTDH Ready-To-Use IHC Kit. MTDH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13860-1-AP targets AEG-1/MTDH in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, RIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | AEG-1/MTDH fusion protein Ag4840 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | metadherin |
| Synonyms | AEG-1/MTDH-Specific, AEG-1, MTDH, Astrocyte elevated gene-1 protein, AEG1 |
| Calculated Molecular Weight | 582 aa, 64 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC045642 |
| Gene Symbol | AEG1/MTDH |
| Gene ID (NCBI) | 92140 |
| RRID | AB_2266734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UE4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| IHC protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| IF protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| IP protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Crohns Colitis MTDH promotes intestinal inflammation by positively regulating TLR signaling. | ||
Oncogene MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo. | ||
Mol Ther Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells. | ||
Oncotarget MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1. | ||
Int J Cancer Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma. |
