• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of MCF-7 using CL488-13860

    IF Staining of MCF-7 using CL488-13860

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 AEG-1/MTDH-Specific antibody (CL488-13860) at dilution of 1:200.

  • FC experiment of HeLa using CL488-13860

    FC experiment of HeLa using CL488-13860

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human AEG-1/MTDH-Specific (CL488-13860) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated AEG-1/MTDH Polyclonal antibody

AEG-1/MTDH Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-13860

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

AEG-1/MTDH-Specific, AEG-1, MTDH, Astrocyte elevated gene-1 protein, AEG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MTDH Ready-To-Use IHC Kit. MTDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13860-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13860 targets AEG-1/MTDH in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 AEG-1/MTDH fusion protein Ag4840 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 metadherin
别名 AEG-1/MTDH-Specific, AEG-1, MTDH, Astrocyte elevated gene-1 protein, AEG1
计算分子量 582 aa, 64 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC045642
基因名称 AEG-1/MTDH
Gene ID (NCBI) 92140
RRIDAB_3672577
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UE4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AEG-1/MTDH antibody CL488-13860Download protocol
FC protocol for CL Plus 488 AEG-1/MTDH antibody CL488-13860Download protocol
Standard Protocols
Click here to view our Standard Protocols