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CoraLite® Plus 488-conjugated AEG-1/MTDH-Specific Polyclonal antibody
AEG-1/MTDH-Specific Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy MTDH Ready-To-Use IHC Kit. MTDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13860-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-13860 targets AEG-1/MTDH-Specific in IF applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse |
Immunogen | AEG-1/MTDH-Specific fusion protein Ag4840 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | metadherin |
Synonyms | 3D3, 3D3/LYRIC, AEG 1, AEG1, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC |
Calculated Molecular Weight | 582 aa, 64 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC045642 |
Gene Symbol | MTDH |
Gene ID (NCBI) | 92140 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86UE4 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 AEG-1/MTDH-Specific antibody CL488-13860 | Download protocol |
FC protocol for CL Plus 488 AEG-1/MTDH-Specific antibody CL488-13860 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |