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验证数据展示

  • IF Staining of mouse heart using CL488-17283

    IF Staining of mouse heart using CL488-17283

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 MYL7 antibody (CL488-17283) at dilution of 1:200.

CoraLite® Plus 488-conjugated MYL7 Polyclonal antibody

MYL7 Polyclonal Antibody for IF-P
Cat No. CL488-17283

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy MYL7 Ready-To-Use IHC Kit. MYL7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17283-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17283 targets MYL7 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen MYL7 fusion protein Ag11058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, light chain 7, regulatory
Synonyms MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a
Calculated Molecular Weight 175 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC027915
Gene Symbol MYL7
Gene ID (NCBI) 58498
RRIDAB_3084002
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01449
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYL7 antibody CL488-17283Download protocol
Standard Protocols
Click here to view our Standard Protocols