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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IMMT Polyclonal antibody

IMMT Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10179

Print datasheet

Synonyms

Mitofilin, MINOS2, MICOS complex subunit MIC60, MIC60, HMP

验证数据展示

  • IF Staining of HepG2 using CL488-10179

    IF Staining of HepG2 using CL488-10179

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Mitofilin antibody (CL488-10179) at dilution of 1:200.

  • FC experiment of HEK-293T using CL488-10179

    FC experiment of HEK-293T using CL488-10179

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Mitofilin (CL488-10179) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy IMMT Ready-To-Use IHC Kit. IMMT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10179-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10179 targets IMMT in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen IMMT fusion protein Ag0102 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inner membrane protein, mitochondrial (mitofilin)
Synonyms Mitofilin, MINOS2, MICOS complex subunit MIC60, MIC60, HMP
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
RRIDAB_2918958
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16891
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IMMT antibody CL488-10179Download protocol
FC protocol for CL Plus 488 IMMT antibody CL488-10179Download protocol
Standard Protocols
Click here to view our Standard Protocols