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验证数据展示

  • IF Staining of HeLa using CL488-12229

    IF Staining of HeLa using CL488-12229

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NDP52 antibody (CL488-12229) at dilution of 1:200.

  • FC experiment of HeLa using CL488-12229

    FC experiment of HeLa using CL488-12229

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NDP52 (CL488-12229) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-12229

    IF Staining of HeLa using CL488-12229

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 NDP52 antibody (CL488-12229) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NDP52 Polyclonal antibody

NDP52 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12229

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12229-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12229 targets NDP52 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen NDP52 fusion protein Ag2866 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium binding and coiled-coil domain 2
Synonyms CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
RRIDAB_3083921
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13137
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NDP52 antibody CL488-12229Download protocol
FC protocol for CL Plus 488 NDP52 antibody CL488-12229Download protocol
Standard Protocols
Click here to view our Standard Protocols