NF-H/NF200 Polyclonal antibody
NF-H/NF200 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 3 publications below |
产品信息
The immunogen of 21471-1-AP is NF-H/NF200 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat, monkey |
Immunogen | NF-H/NF200 fusion protein Ag15249 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | neurofilament, heavy polypeptide |
Synonyms | 200 kDa neurofilament protein, NEFH, Neurofilament H, NF, NF H, NF200, NFH, NF-H |
Calculated Molecular Weight | 1026 aa, 112 kDa |
Observed Molecular Weight | 200 kDa |
GenBank Accession Number | BC073969 |
Gene Symbol | NF-H |
Gene ID (NCBI) | 4744 |
RRID | AB_10734324 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P12036 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NF-H/NF200 antibody 21471-1-AP | Download protocol |
IHC protocol for NF-H/NF200 antibody 21471-1-AP | Download protocol |
FC protocol for NF-H/NF200 antibody 21471-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mater Today Bio Ropivacaine microsphere-loaded electroconductive nerve dressings for long-acting analgesia and functional recovery following diabetic peripheral nerve injury | ||
Sci Total Environ Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons. | ||
iScience Synthetic amyloid-β oligomers drive early pathological progression of Alzheimer's disease in nonhuman primates. | ||
Toxicol Appl Pharmacol Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways. | ||
Plast Reconstr Surg Glob Open A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model. |