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- KD/KO Validated
NLRX1 Polyclonal antibody
NLRX1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, HeLa cells, mouse colon tissue, mouse heart tissue, THP-1 cells, rat colon tissue, rat heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human heart tissue, human liver tissue, mouse heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17215-1-AP targets NLRX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | NLRX1 fusion protein Ag11000 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | NLR family member X1 |
Synonyms | Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9 |
Calculated Molecular Weight | 975 aa, 108 kDa |
Observed Molecular Weight | 100-110 kDa |
GenBank Accession Number | BC013199 |
Gene Symbol | NLRX1 |
Gene ID (NCBI) | 79671 |
RRID | AB_2236031 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86UT6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for NLRX1 antibody 17215-1-AP | Download protocol |
IHC protocol for NLRX1 antibody 17215-1-AP | Download protocol |
IF protocol for NLRX1 antibody 17215-1-AP | Download protocol |
IP protocol for NLRX1 antibody 17215-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.
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J Exp Med NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.
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Front Immunol NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages. |