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  • KD/KO Validated

NLRX1 Polyclonal antibody

NLRX1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17215-1-AP

Print datasheet

Synonyms

Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, HeLa cells, mouse colon tissue, mouse heart tissue, THP-1 cells, rat colon tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human liver tissue, mouse heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17215-1-AP targets NLRX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NLRX1 fusion protein Ag11000 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NLR family member X1
Synonyms Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9
Calculated Molecular Weight 975 aa, 108 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC013199
Gene Symbol NLRX1
Gene ID (NCBI) 79671
RRIDAB_2236031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NLRX1 antibody 17215-1-APDownload protocol
IHC protocol for NLRX1 antibody 17215-1-APDownload protocol
IF protocol for NLRX1 antibody 17215-1-APDownload protocol
IP protocol for NLRX1 antibody 17215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Mitochondrial DNA stress primes the antiviral innate immune response.

Authors - A Phillip West
humanWB

Mol Cell

Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.

Authors - Samuel A Killackey
  • KD Validated
humanWB,IHC

J Exp Med

NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.

Authors - Geurt Stokman
  • KO Validated
human,mouseWB

PLoS Pathog

PMID: 28542569

Authors - Jae-Hoon Kim
humanWB

Front Immunol

PMID: 30344524

Authors - Tünde Fekete
mouseIF

Front Immunol

NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.

Authors - Juin-Hua Huang