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Glucocorticoid receptor Polyclonal antibody

Glucocorticoid receptor Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 24050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

NR3C1, GRL, GR, GCR, GCCR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells
Positive IP detected inHepG2 cells, mouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24050-1-AP targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, zebrafish, rare minnow
免疫原 Glucocorticoid receptor fusion protein Ag21146 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
别名 NR3C1, GRL, GR, GCR, GCCR
计算分子量 86 kDa
观测分子量 94-97 kDa
GenBank蛋白编号BC015610
基因名称 Glucocorticoid receptor
Gene ID (NCBI) 2908
RRIDAB_2813890
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04150
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

实验方案

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IHC protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IF protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IP protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.

Authors - Wenxiang Hu
mouseIHC

Cell Metab

A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.

Authors - Anne Loft
humanWB

Sci Adv

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Authors - Leiming Wang
mousechIP

Mol Cell

Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.

Authors - Fabiana Quagliarini
mouseWB,ChIP,IP

J Clin Invest

REVERBa couples the circadian clock to hepatic glucocorticoid action.

Authors - Giorgio Caratti
mouseChIP

Mol Psychiatry

The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.

Authors - Alexander S Häusl
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