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- KD/KO Validated
CoraLite® Plus 488-conjugated Glucocorticoid receptor Polyclonal antibody
Glucocorticoid receptor Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24050-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
The immunogen of CL488-24050 is Glucocorticoid receptor Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | Glucocorticoid receptor fusion protein Ag21146 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
| Synonyms | GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1 |
| Calculated Molecular Weight | 86 kDa |
| Observed Molecular Weight | 94-97 kDa |
| GenBank Accession Number | BC015610 |
| Gene Symbol | NR3C1 |
| Gene ID (NCBI) | 2908 |
| RRID | AB_2919198 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04150 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-24050 | Download protocol |
| FC protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-24050 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol Hypothalamic AgRP neurons exert top-down control on systemic TNF-α release during endotoxemia |
