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验证数据展示

  • FC experiment of HeLa using CL488-66878

    FC experiment of HeLa using CL488-66878

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Nurr1/NR4A2 Monoclonal antibody (CL488-66878, Clone:1H5B4)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Nurr1/NR4A2 Monoclonal antibody

Nurr1/NR4A2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66878

Print datasheet

CloneNo. 1H5B4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC (Intra)

Synonyms

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NR4A2 Ready-To-Use IHC Kit. NR4A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66878-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66878 targets Nurr1/NR4A2 in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse
Immunogen Nurr1/NR4A2 fusion protein Ag1418 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear receptor subfamily 4, group A, member 2
Synonyms HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC009288
Gene Symbol Nurr1
Gene ID (NCBI) 4929
RRIDAB_2934503
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43354
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Nurr1/NR4A2 antibody CL488-66878Download protocol
Standard Protocols
Click here to view our Standard Protocols