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验证数据展示

  • FC experiment of HeLa using CL488-66878

    FC experiment of HeLa using CL488-66878

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Nurr1/NR4A2 Monoclonal antibody (CL488-66878, Clone:1H5B4)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Nurr1/NR4A2 Monoclonal antibody

Nurr1/NR4A2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66878

产品说明书

CloneNo. 1H5B4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

FC (Intra)

别名

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NR4A2 Ready-To-Use IHC Kit. NR4A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66878-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66878 targets Nurr1/NR4A2 in FC (Intra) applications and shows reactivity with Human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse
免疫原 Nurr1/NR4A2 fusion protein Ag1418 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear receptor subfamily 4, group A, member 2
别名 HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR
计算分子量 66 kDa
观测分子量 67 kDa
GenBank蛋白编号BC009288
基因名称 Nurr1
Gene ID (NCBI) 4929
RRIDAB_2934503
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP43354
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Nurr1/NR4A2 antibody CL488-66878Download protocol
Standard Protocols
Click here to view our Standard Protocols