验证数据展示
经过测试的应用
Positive FC (Intra) detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-10724 targets NRAS in FC (Intra) applications and shows reactivity with human samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | NRAS fusion protein Ag1081 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | neuroblastoma RAS viral (v-ras) oncogene homolog |
别名 | HRAS1, Transforming protein N Ras, NRAS1, N ras, GTPase NRas |
计算分子量 | 21 kDa |
观测分子量 | 21 kDa |
GenBank蛋白编号 | BC005219 |
基因名称 | NRAS |
Gene ID (NCBI) | 4893 |
RRID | AB_3084523 |
偶联类型 | CoraLite® Plus 555 Fluorescent Dye |
最大激发/发射波长 | 554 nm / 570 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P01111 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML).
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 555 NRAS antibody CL555-10724 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |