验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66150 targets NSE in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | NSE fusion protein Ag19106 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | enolase 2 (gamma, neuronal) |
| 别名 | ENO2, Gamma enolase, Enolase 2, EC:4.2.1.11, 6F8G3 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC002745 |
| 基因名称 | NSE |
| Gene ID (NCBI) | 2026 |
| RRID | AB_2883272 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P09104 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NSE antibody CL488-66150 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |