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验证数据展示

  • IF Staining of HepG2 using CL488-66335

    IF Staining of HepG2 using CL488-66335

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66335 (NUDT21 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NUDT21 Monoclonal antibody

NUDT21 Monoclonal Antibody for IF/ICC
Cat No. CL488-66335

Print datasheet

CloneNo. 1C4C8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, DKFZp686H1588, Nudix motif 21, NUDT21

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66335-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66335 targets NUDT21 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen NUDT21 fusion protein Ag12260 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Synonyms CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, DKFZp686H1588, Nudix motif 21, NUDT21
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001403
Gene Symbol NUDT21
Gene ID (NCBI) 11051
RRIDAB_2883312
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43809
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NUDT21 antibody CL488-66335Download protocol
Standard Protocols
Click here to view our Standard Protocols