Nestin Monoclonal antibody

Nestin Monoclonal Antibody for WB, ELISA
Cat No. 66259-1-Ig

产品说明书

CloneNo. 1D11H2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, ELISA

NES, Nestin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inNeuro-2a cells, HEK-293 cells, PC-12 cells, rat cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy Nestin Ready-To-Use IHC Kit. Nestin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66259-1-Ig targets Nestin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nestin
别名 NES, Nestin
计算分子量 177 kDa
观测分子量 150-170 kDa
GenBank蛋白编号NM_006617
基因名称 Nestin
Gene ID (NCBI) 10763
RRIDAB_2756523
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48681
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

实验方案

Product Specific Protocols
WB protocol for Nestin antibody 66259-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
human

Cell Res

RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability.

Authors - Feng Xiong
mouseFC

J Allergy Clin Immunol

Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1.

Authors - Xia Ke
rat

Small

Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.

Authors - Xiheng Lu
mouseWB

EBioMedicine

Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.

Authors - Guangtian Wang
rat

Int J Biol Macromol

A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects

Authors - Wentao Shi
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