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验证数据展示

  • WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

Nestin Monoclonal antibody, PBS Only

Nestin Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66259-1-PBS

产品说明书

CloneNo. 1D11H2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

NES, Nestin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Nestin Ready-To-Use IHC Kit. Nestin primary antibody included.

产品信息

66259-1-PBS targets Nestin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nestin
别名 NES, Nestin
计算分子量 177 kDa
观测分子量 150-170 kDa
GenBank蛋白编号NM_006617
基因名称 Nestin
Gene ID (NCBI) 10763
RRIDAB_2756523
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48681
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.