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Conjugate
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验证数据展示

  • WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

Nestin Monoclonal antibody, PBS Only

Nestin Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66259-1-PBS

产品说明书

CloneNo. 1D11H2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

NES, Nestin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Nestin Ready-To-Use IHC Kit. Nestin primary antibody included.

产品信息

66259-1-PBS targets Nestin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

fusion protein

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nestin
别名 NES, Nestin
计算分子量 177 kDa
观测分子量 150-170 kDa
GenBank蛋白编号NM_006617
基因名称 Nestin
Gene ID (NCBI) 10763
RRIDAB_2756523
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP48681
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.