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Nestin Monoclonal antibody, PBS Only

Nestin Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11H2

Cat no : 66259-1-PBS

Print datasheet

Synonyms

NES, Nestin

验证数据展示

  • WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Nestin Ready-To-Use IHC Kit. Nestin primary antibody included.

产品信息

66259-1-PBS targets Nestin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nestin
Synonyms NES, Nestin
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 150-170 kDa
GenBank Accession NumberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_2756523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48681
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.