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验证数据展示

  • WB analysis using 26975-1-AP (same clone as 26975-1-PBS)

    WB analysis using 26975-1-AP (same clone as 26975-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

  • WB analysis using 26975-1-AP (same clone as 26975-1-PBS)

    WB analysis using 26975-1-AP (same clone as 26975-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 26975-1-AP (same clone as 26975-1-PBS)

    IF Staining of rat brain using 26975-1-AP (same clone as 26975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

  • IF Staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    IF Staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    IF Staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    IF Staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

  • FC experiment of U-87 MG using 26975-1-AP (same clone as 26975-1-PBS)

    FC experiment of U-87 MG using 26975-1-AP (same clone as 26975-1-PBS)

    1X10^6 U-87 MG cells were intracellularly stained with 0.2 ug Anti-Human NeuN (26975-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    IHC staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IHC staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    IHC staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IF Staining of mouse brain using 26975-1-AP (same clone as 26975-1-PBS)

    IF Staining of mouse brain using 26975-1-AP (same clone as 26975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

NeuN Polyclonal antibody, PBS Only

NeuN Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA
Cat No. 26975-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

别名

Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.

产品信息

26975-1-PBS targets NeuN in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag25689

Product name: Recombinant human NeuN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001082575

Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hexaribonucleotide binding protein 3
别名 Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen
观测分子量 46-52 kDa
GenBank蛋白编号NM_001082575
基因名称 NeuN
Gene ID (NCBI) 146713
RRIDAB_2880708
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA6NFN3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).


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